MABF520

Anti-CD11b Antibody (human/mouse), APC, clone M1/70

Name: Anti-CD11b Antibody (human/mouse), APC, clone M1/70
Brand: MM

clone M1/70, 0.2 mg/mL, from rat

Synonym(s):

Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD antigen CD11b

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-CD11b Antibody (human/mouse), APC, clone M1/70, clone M1/70, 0.2 mg/mL, from rat

biological source

rat

conjugate

Allophycocyanin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

M1/70, monoclonal

species reactivity

mouse, human

packaging

antibody small pack of 25 μg

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2bκ

UniProt accession no.

P05555
P11215

shipped in

wet ice

target post-translational modification

unmodified

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD11b in one million C57BI/6 bone marrow cells.

Application

Research Category
Inflammation & Immunology

This rat monoclonal antibody Anti-CD11b (human/mouse), APC & clone M1/70 validated for use in IP;IHC;Flow Cytometry;Immunofluorescence for the detection of CD11b.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The M1/70 antibody reacts with human and mouse CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells. The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.

Immunogen

Immunogen information for this antibody is proprietary information.

Physical form

Purified rat monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Flow Cytometry Analysis of SIRT6 Expression in Peritoneal Macrophages.

P??rez-Torrado, et al.

Bio-protocol, 12 (2022)

Innate Immune Functions of Astrocytes are Dependent Upon Tumor Necrosis Factor-Alpha.

Kyla R Rodgers et al.

Scientific reports, 10(1), 7047-7047 (2020-04-29)

Acute inflammation is a key feature of innate immunity that initiates clearance and repair in infected or damaged tissues. Alternatively, chronic inflammation is implicated in numerous disease processes. The contribution of neuroinflammation to the pathogenesis of neurological conditions, including infection

A novel CX3CR1 inhibitor AZD8797 facilitates early recovery of rat acute spinal cord injury by inhibiting inflammation and apoptosis.

Guozhao Chen et al.

International journal of molecular medicine, 45(5), 1373-1384 (2020-04-24)

The present study aimed to evaluate the effect of the CX3CR1 inhibitor AZD8797 in early recovery after acute SCI and elucidate its potential mechanism in blocking inflammation and apoptosis. Adult rats were sacrificed after 3, 7, 10, or 14 days of

Wei Wang et al.

Immunity, 51(6), 1102-1118 (2019-11-24)

Young children are more susceptible to developing allergic asthma than adults. As neural innervation of the peripheral tissue continues to develop after birth, neurons may modulate tissue inflammation in an age-related manner. Here we showed that sympathetic nerves underwent a

Global Trade Item Number

SKU	GTIN
MABF520	04053252962585

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