Anti-Aldolase C Antibody, N-Term, clone 4A9

Fructose-bisphosphate aldolase C (EC 4.1.2.13; UniProt P09972; also known as Aldolase 3, Brain-type aldolasefructoaldolase C, Fructose-1,6-biphosphate triosephosphate lyase) is encoded by the ALDOC (also known as ALDC) gene (Gene ID 230) in human. Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Three aldolase genes (ALDOA, ALDOB, ALDOC) are found in mammals that encode aldolase A, B, and C. Aldolase A is generally considered to be a muscle enzyme, aldolase B a liver enzyme, while aldolase C shows tissue-specific expression in adult brain with low activity in fetal tissues. Aldolase C expression is found in gray matter astrocytes and cells of the pia mater. Aldolase A and C mRNA expressions are mutually exclusive in murine central nervous system by in situ hybridization analysis, with aldolase A mRNA being detected in neurons and aldolase C in astrocytes, Purkinje cells, and some cells of the pia mater. Aldolase C can therefore be a useful marker of astrocytes, although Purkinje cells of the cerebellum also contain high levels of aldolase C.

~39 kDa observed

Epitope: Near N-terminus, 20 amino acids of aldolase C protein

Recombinant protein corresponding to human Aldolase C near the N-terminus.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Aldolase C Antibody, N-Term, clone 4A9 is validated for use in Western Blotting, Immunocytochemistry for the detection of Aldolase C.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Aldolase C in 10 µg of human and mouse brain tissue lysate.
Western Blotting Analysis: A representative lot detected Aldolase C in bovine cerebellum tissue lysate.
Immunocytochemistry: A representative lot selectively stained Aldolase C-positive astrocytes in mixed neuron/glial cultures.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing PBS with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Aldolase C in 10 µg of rat brain tissue lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.