Anti-Phosphotyrosine, clone RM111 Antibody

N/A kDa observed

The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

recombinant protein corresponding to All Phosphotyrosine Protein.

Anti-Phosphotyrosine, clone RM111 is an antibody against Phosphotyrosine for use in WB, ICC, IP, IH.

Immunoprecipitation Analysis: 1:1000 dilution of this antibody immunoprecipitated phosphotyrosine modified proteins from EGF-treated A431 cell lysate
Immunocytochemistry Analysis: 1:500 dilution of this antibody detected phosphotyrosine modified proteins from serum-starved A431 cells treated with EGF
Immunohistochemistry Analysis: 1:500 dilution of this antibody detected phosphotyrosine modified proteins from human lung cancer tissue
Luminex Analysis: This antibody demonstrated to only react with phosphorylated tyrosine when tested against a phospho and non-phospho amino acid panel

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Protein A purified

Purified rabbit monoclonal IgG in PBS buffer containing 1% BSA and 0.09% sodium azide

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting on EGF treated Cos7 cell lysate
Western Blotting Analysis: 1:1000 of this antibody detected phosphotyrosine modified proteins from 10 µg of EGF treated Cos7 cell lysate

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.