Anti-PP2C Isoform delta Antibody, clone 2F7.1

Evaluated by Western Blotting in HL-60 human promyelocytic leukemia cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PP2C-delta in 10 µg of HL-60 human promyelocytic leukemia cell lysate.

Anti-PP2C Isoform delta Antibody, clone 2F7.1, Cat. No. MABS1189, is a highly specific mouse monoclonal antibody, that targets PPM1D and has been tested in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PP2C-delta in human prostate cancer, normal human brain and rat testis tissue sections.

Research Category
Epigenetics & Nuclear Function

Clone 2F7.1 targets an internal epitope conserved among the two human spliced isoforms and mouse/rat PP2C-delta (Uniprot O15297, B1WCA0, Q9QZ67).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Protein phosphatase 1D (EC 3.1.3.16; UniProt O15297; also known as Wild-type p53-induced protein phosphatase 1, PP2C-delta, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta) is encoded by the PPM1D (also known as WIP1) gene (Gene ID 8493) in human. Wild-type p53-induced phosphatase 1 (WIP1) or PP2C-delta is a serine/threonine phosphatase. Unlike PP2A or PP1 enzymes, WIP1 functions as a monomer whose catalytic activity is dependents on Mg2+ or Mn2+ and is insensitive to inhibition by okadaic acid or microcystin. Casein kinase 2 (CK2) andPP2C-delta catalyze the phosphorylation and dephosphorylation, respectively, of LSD1 on Ser131 and Ser137 residues. S131/S137 phosphorylation promots LSD1 interaction with RNF168 and RNF168-dependent 53BP1 ubiquitination and subsequent recruitment to DNA damage sites. Other known PP2C-delta targets include ATM (S367, S1981), -H2AX, CHK1 (S345) and CHK2 (T68).

~60 kDa observed. 66.68/47.21 kDa (human isoform 1/2), 65.72 kDa (mouse), and 65.68 kDa (rat) calculated.

GST-tagged recombinant human PP2C-delta internal fragment.

Concentration: Please refer to lot specific datasheet.

Format: Purified

Protein G purified.

Purified mouse monoclonal antibody IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Stable for 1 year at 2-8°C from date of receipt.