MABS451
Anti-Linear Ubiquitin Antibody, clone LUB9
clone LUB9, from mouse
Synonym(s):
Polyubiquitin-C, Ubiquitin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Linear Ubiquitin Antibody, clone LUB9, clone LUB9, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
LUB9, monoclonal
species reactivity
human
technique(s)
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... UBC(7316)
Application
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Anti-Linear Ubiquitin Antibody, clone LUB9 | MABS451 is an antibody against Linear Ubiquitin Antibody for use in Western Blotting.
Research Category
Signaling
Signaling
Western Blotting Analysis: A representative lot detected Linear Ubiquitin in (M1-linked) ubiquitin chains (Yoshiteru, S. et al. (2013). EMBO. 32:2463-2476).
Analysis Note
Evaluated by Western Blotting in recombinant protein Linear Polyubiquitin chains, K48 Ub 2-7, K63 Ub 2-7, and K11-only Ubn-ubiquitinylated HM substrate protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of Linear Polyubiquitin recombinant protein chains, bands were not detected in K48 Ub 2-7, K63 Ub 2-7, and K11-only Ubn-ubiquitinylated HM substrate protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of Linear Polyubiquitin recombinant protein chains, bands were not detected in K48 Ub 2-7, K63 Ub 2-7, and K11-only Ubn-ubiquitinylated HM substrate protein.
Biochem/physiol Actions
This antibody detects only Linear Ubiquitin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Multiple bands observed
Ubiquitin functions as a marker to indicate the fate of other proteins in the cell. It can be attached as a single molecule or as a polyubiquitin chain. Although ubiquitinated proteins are oftentimes tagged for degradation by the 20S proteosome, ubiquitination can also indicate alternative fates depending on the lysine residue that is involved in linking the ubiquitin chain to the target protein. Ubiquitin chains linked by Lys33 to kinase substrates such as AMP-activated protein kinases, (AMPKα1, BRSK1, MARK3, MARK4, and NUAK1), may activate or deactivate these proteins, supporting the role for uniquitin in regulating signaling.
Immunogen
Ovalbumin-conjugated linear peptide corresponding to human Linear Ubiquitin.
Other Notes
Concentration: Please refer to lot specific datasheet.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Xiangqin Kuang et al.
Cellular & molecular biology letters, 28(1), 62-62 (2023-08-01)
Bladder cancer (BLCA) is a malignancy that frequently metastasizes and leads to poor patient prognosis. It is essential to understand the molecular mechanisms underlying the progression and metastasis of BLCA and identify potential biomarkers. The expression of peptidase inhibitor 16
Daisuke Oikawa et al.
Frontiers in immunology, 11, 601926-601926 (2020-12-18)
The LUBAC ubiquitin ligase complex, composed of the HOIP, HOIL-1L, and SHARPIN subunits, stimulates the canonical nuclear factor-κB (NF-κB) activation pathways through its Met1-linked linear ubiquitination activity. Here we performed cellular and mathematical modeling analyses of the LUBAC involvement in
Hirohisa Miyashita et al.
Frontiers in immunology, 12, 635475-635475 (2021-04-06)
Nuclear dot protein 52 kDa (NDP52, also known as CALCOCO2) functions as a selective autophagy receptor. The linear ubiquitin chain assembly complex (LUBAC) specifically generates the N-terminal Met1-linked linear ubiquitin chain, and regulates innate immune responses, such as nuclear factor-κB
Seshiru Nakazawa et al.
Oncology letters, 24(5), 394-394 (2022-10-25)
The linear ubiquitin chain assembly complex (LUBAC), which is composed of RING finger protein 31 (RNF31), RANBP2-type and C3HC4-type zinc finger containing 1 and SHANK-associated RH domain interactor subunits, is the only ubiquitin ligase to generate Met1-linked linear ubiquitin chains.
Hayley I Muendlein et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(24), e2113872119-e2113872119 (2022-06-07)
ZBP1 is widely recognized as a mediator of cell death for its role in initiating necroptotic, apoptotic, and pyroptotic cell death pathways in response to diverse pathogenic infection. Herein, we characterize an unanticipated role for ZBP1 in promoting inflammatory responses
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABS451 | 04055977125375 |
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