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Merck

MABT106

Anti-Protein Spindly Antibody, clone 9D10.2

clone 9D10.2, from mouse

Synonym(s):

Protein Spindly, hSpindly, Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99, Rhabdomyosarcoma antigen MU-RMS-40.4A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Protein Spindly Antibody, clone 9D10.2, clone 9D10.2, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

9D10.2, monoclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

NP_060255

UniProt accession no.

Q96EA4

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... SPDL1(54908)

Analysis Note

Control
HEK293 cell lysate
Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Protein Spindly in 10 µg of HEK293 cell lysate.

Application

Detect Protein Spindly using this Anti-Protein Spindly Antibody, clone 9D10.2 validated for use in ICC, Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Protein Spindly in HeLa and NIH/3T3.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein spindly, otherwise known as the coiled-coil domain-containing protein 99 (CCDC99), is a phosphoprotein that regulates mitosis. It interacts with the Rod/ZW10/Zwilch complex, and is involved in recruiting, docking, and alignment of motor protein dynein at mitotic kinetochores. Spindly may also be involved in checkpoint signaling and microtubule attachment.
~70 kDa observed. This protein is known to exist in two isoforms at 70 kDa and 56 kDa An uncharacterized band may be observed at ~38 kDa in some cell lysates.

Immunogen

GST-tagged recombinant protein corresponding to human Protein Spindly.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bingteng Xie et al.
Cell research, 28(4), 462-475 (2018-02-22)
Before fertilization, mammalian oocyte undergoes an asymmetric division which depends on eccentric positioning of the spindle at the oocyte cortex to form a polar body and an egg. Since the centriole is absent and, as a result, the polar array

Global Trade Item Number

SKUGTIN
MABT10604053252551130

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