MABT161
Anti-Versican Antibody, clone 8D8.1
clone 8D8.1, from mouse
Synonym(s):
Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Chondroitin sulfate proteoglycan 2, Glial hyaluronate-binding protein, GHAP, Large fibroblast proteoglycan, PG-M
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
8D8.1, monoclonal
Application:
IHC, WB
Species reactivity:
human, mouse
Citations:
3
Technique(s):
immunohistochemistry: suitable, western blot: suitable
Uniprot accession no.:
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
8D8.1, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgMκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Vcan(13003)
Analysis Note
Control
Human thalamus tissue lysate
Human thalamus tissue lysate
Evaluated by Immunohistochemistry in human thalamus tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human thalamus tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human thalamus tissue lysate.
Application
Detect Versican using this mouse monoclonal antibody, Anti-Versican Antibody, clone 8D8.1 validated for use in IHC & western blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human cerebellum, and mouse brain and embryonic skeletal muscle.
Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Versican in mouse leg muscle tissue lysate.
Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Versican in mouse leg muscle tissue lysate.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
This antibody recognizes the C-terminus of Veriscan.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
372 kDa calculated. Uniprot describes three isoforms at 265 kDa, 182 kDa, and 74 kDa Versican is known to be secreted following valvular interstitual cell (VIC) injury and repair (Carthy, J., et al. (2012). Cardiovac. Pathol. 21(2):74-82.).
Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan. Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human Veriscan.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lulu Xu et al.
Molecular medicine reports, 13(6), 5005-5012 (2016-04-26)
The versican family is important in the modulation of inflammation, however, the role of versican V1 (V1) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying mechanisms remain to be elucidated. To investigate this, the present study performed experiments
Expression of a novel versican variant in dorsal root ganglia from spared nerve injury rats.
Oliver Bogen et al.
Molecular pain, 15, 1744806919874557-1744806919874557 (2019-08-21)
Suguru Yamasaki et al.
iScience, 25(1), 103657-103657 (2022-01-14)
Pluripotent stem cell (PSC)-derived retinal sheet transplanted in vivo can form structured photoreceptor layers, contact with host bipolar cells, and transmit light signals to host retinas. However, a major concern is the presence of graft bipolar cells that may impede host-graft
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABT161 | 04053252917004 |
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