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Merck

MABT379

Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1

clone 5F8.1, from mouse

Synonym(s):

Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1, clone 5F8.1, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5F8.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_060513

UniProt accession no.

Q5XXA6

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ANO1(55107)

Analysis Note

Evaluated by Western Blotting in PC-3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Anoctamin-1/TMEM16A in 10 µg of PC-3 cell lysate.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Anoctamin-1/TMEM16A in human prostate cancer sections.
This Anti-Anoctamin-1/TMEM16A Antibody, clone 5F8.1 is validated for use in western blotting & IHC for the detection of Anoctamin-1/TMEM16A.

Biochem/physiol Actions

This antibody recognizes isoforms 1 and 2 of Anoctamin-1 based upon immunogen design.

General description

Discovered on gastrointestinal stromal tumors protein 1 (Dog1), also known as Anoctamin-1/TMEM16A, is a novel multi-pass membrane protein belonging to the anoctamin family. Anoctamin-1/TMEM16A functions as a Ca2+-activated Cl- channel and is a required component of tracheal development. Expression of Anoctamin-1/TMEM16A is elevated in the gastrointestinal stromal tumors (GISTs), KIT signaling-driven mesenchymal tumors of the GI tract. It is also expressed at more moderate levels in epithelia from foregut, kidney, lung, pancreas and salivary glands. Recent studies suggest that it may serve a roll as a diagnostic marker in the classification of GISTs.
~114 kDa and ~98 kDa observed. Three isoforms are known to exist at 114 kDa (isoform 1), 98 kDa (isoform 2), and 74 kDa (isoform 3).

Immunogen

Epitope: N-terminal cytoplasmic domain
GST-tagged recombinant protein corresponding to the N-terminal cytoplasmic domain of human Anoctamin-1/TMEM16A.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MABT37904053252955792

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