MABT419
Anti-CD2AP Antibody, clone 2A2.1
clone 2A2.1, from mouse
Synonym(s):
CD2-associated protein, CD2AP, Adapter protein CMS, Cas ligand with multiple SH3 domains
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2A2.1, monoclonal
Application:
IHC, WB
Citations:
1
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2A2.1, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CD2AP(23607)
General description
CD2-associated protein (UniProt Q9Y5K6; also known as Adapter protein CMS, Cas ligand with multiple SH3 domains) is encoded by the CD2AP (also known as CMS) gene (Gene ID 23607) in human. CD2AP is an adapter protein expressed in all tissues except brain. CD2AP harbors three SH3 domains at its N-terminal, a proline-rich domain, and a C-terminal leucine-zipper domain that mediates CD2AP homodimerization. CD2AP is known to interact with actin-associated proteins, such as cortactin, and actin capping protein CAPZ. The SH3 domains of CD2AP mediate its association to the hypervariable C terminal region of Rac1, allowing CD2AP to act as a linker between Rac1 and the actin-binding proteins cortactin and CAPZ. CD2AP also plays an important role in the formation of the slit diaphragm in the kidney glomerulus between podocyte foot processes by linking the cell-cell adhesion protein nephrin to the cytoskeleton. CD2AP gene mutations are associated with kidney diseases such as sporadic nephrotic syndrome and focal segmental glomerulosclerosis.
~71 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to the C-terminal half of human CD2AP.
Application
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected CD2AP in human kidney and human colon tissue.
This Anti-CD2AP Antibody, clone 2A2.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of CD2AP.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD2AP in 10 µg of Raji cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD2AP in 10 µg of Raji cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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