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Merck

OP14L

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

lyophilized, clone 9G6, Calbiochem®

Synonym(s):

Anti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Product Name

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6), lyophilized, clone 9G6, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

9G6, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Frozen Sections (5 µg/mL)
Immunoprecipitation (1 µg/sample)
Paraffin Sections )

isotype

IgG1

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ERBB2(2064)

Analysis Note

Negative Control
HepG2 cells or normal skin
Positive Control
SK-BR-3 cells or breast carcinoma tissue

Application

Frozen Sections (5 µg/ml)

Immunoprecipitation (1 µg/sample)

Paraffin Sections (see application references)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.
Recognizes the ~185 kDa c-ErbB2/c-Neu in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.

Immunogen

Human
cells overexpressing human c-ErbB2/c-Neu

Other Notes

Immunoprecipitates the ~185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein. This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Van De Vijver, M.J., et al.1988 New Engl. J. Med.319, 1239.
DiFiore, P.P., et al.1987 Science237, 178.
Slamon, D.J., et al. 1987 Science235, 177.
Varley, J.M., et al.1987 Oncogene1, 423.
Bargmann, C.I., et al. 1986 Nature319, 226.
Yamamoto, T., et al.1986 Nature319, 230.
Blick, M., et al. 1984 Blood64, 1234.

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Preparation Note

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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