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Merck

OP97

Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2)

liquid, clone 22C2, Calbiochem®

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About This Item

UNSPSC Code:
12352203

Product Name

Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2), liquid, clone 22C2, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

22C2, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... MTOR(2475)

Analysis Note

Positive Control
Jurkat or HeLa cells

Application


Immunoblotting (1.7 g/ml, see application references)
Immunoprecipitation (1-10 g/ml, see application references)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SpAG8 mouse myeloma cells. Recognizes the ~290 kDa mTOR/FRAP protein.
Recognizes the ~290 kDa mTOR protein in HEK293 and Jurkat cells.
This Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) is validated for use in Immunoblotting, Immunoprecipitation for the detection of mTOR/FRAP (Ab-2).

Immunogen

Human
a synthetic peptide corresponding to amino acids 230-240 of human TOR

Other Notes

Kimura N et al. 2003. Genes Cells8, 65.
Sekulic A et al. 2000. Cancer Res.60, 3504.
Hosoi, H. 1999. Cancer Res.59, 886.
Alarcon, C.M., et al. 1996. Genes Dev.10, 279.
Freeman, K., and Livi, G.P. 1996. Gene172, 143.
Hosoi, H., et al. 1996. AACR 87th Ann. Mtg. Abstract 3445.
Cardenas, M.E., and Heitman, J. 1995. EMBO J.14, 5892.
Lorenz, M.C., and Heitman, J. 1995. J. Biol. Chem.270, 27531.
Sabatini, D.M., et al. 1995. J. Biol. Chem.270, 20875.
Zheng, X.F., et al. 1995. Cell82, 121.
Brown, E.J., et al. 1994. Nature369, 756.
Sabatini, D.M., et al. 1994. Cell78, 35.
Stan, R., et al. 1994. J. Biol. Chem.269, 32027.
Kunz, J., et al. 1993. Cell73, 585.
Heitman, J., et al. 1991. Science253, 905.
The immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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wgk

WGK 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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