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Merck

PCYTMAG-23K

Millipore

MILLIPLEX® Porcine Cytokine and Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology in porcine serum, plasma and cell culture samples.

Synonym(s):

luminex porcine cytokine chemokine growth factor multiplex assay, luminex porcine pro inflammatory anti inflammatory cytokine panel, millipore porcine inflammation multiplex kit, porcine interleukin immunoassay panel

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

pig

manufacturer/tradename

Milliplex®

assay range

accuracy: 92-103%
standard curve range: 0.005-20 ng/mL
(IL-1α)
standard curve range: 0.012-50 ng/mL
(IL-8)
standard curve range: 0.024-100 ng/mL
(GM-CSF, IL-1ra, IL-2, IL-6, IL-10, IL-12, IL-18 & TNFα)
standard curve range: 0.061-250 ng/mL
(IL-4)
standard curve range: 0.122-500 ng/mL
(IFNγ & IL-1β)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

With a similarity to humans in heart size, blood flow rate, skin, liver enzymes and bone healing, porcine models have replaced many canine and non-human primate models in many research areas such as cardiovascular, toxicology, pharmacology, aging and bone. “Minipigs,” bred to retain the similar heart size and function as humans, but age without growing too large, have been used for long term preclinical or multi-generational studies which may be performed in a comparatively short period of time.

MILLIPLEX® Porcine Cytokine/Chemokine Panel can be used for the simultaneous quantification of any or all of the following analytes in porcine tissue/cell lysate and culture supernatant samples and serum or plasma samples: GM-CSF, IFNγ, IL-1α, IL-1ra, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

  • Analytes: GM-CSF, IFNγ, IL-1α, IL-1β, IL-1Ra, IL-2, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-12, IL-18, TNF-α
  • Recommended Sample Type: serum, plasma or cell/tissue culture supernatants or lysate
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • NOTE: Extra centrifugation and attention may be needed for samples with extremely high lipid content.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C.
  • Research Category: Inflammation & Immunology

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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David S Gardner et al.
American journal of physiology. Renal physiology, 310(4), F259-F271 (2015-11-27)
Acute kidney injury (AKI) is a common and serious condition with no specific treatment. An episode of AKI may affect organs distant from the kidney, further increasing the morbidity associated with AKI. The mechanism of organ cross talk after AKI
Ruipeng Zhao et al.
International immunopharmacology, 99, 107905-107905 (2021-07-10)
To determine whether idealized anterior cruciate ligament reconstruction (IACL-R) restores normal gait features, and whether inflammatory factors are involved in the pathogenesisof post-traumatic osteoarthritis (PTOA). Fourteen mature female minipigs were allocated to a sham group (n = 7) or an IACL-R group
Azita Mellati et al.
Frontiers in immunology, 13, 850271-850271 (2022-06-21)
Ischaemia-reperfusion injury (IRI) is an inevitable process in transplantation and results in inflammation and immune system activation. Alpha-1 antitrypsin (AAT) has anti-inflammatory properties. Normothermic machine perfusion (NMP) can be used to deliver therapies and may help in assessing the effects
Turun Song et al.
American journal of translational research, 13(8), 8965-8976 (2021-09-21)
Dyslipidemia aggravates kidney injury distal to atherosclerotic renal artery stenosis (ARAS). Besides dyslipidemia, metabolic syndrome (MetS) also involves development of obesity and insulin-resistance (IR). We hypothesized that concurrent obesity and IR magnify swine stenotic-kidney damage beyond dyslipidemia. Pigs with unilateral
Christina Veit et al.
Frontiers in veterinary science, 7, 617634-617634 (2021-02-16)
Poor health is a risk factor for damaging behaviors, but the mechanisms behind this link are unknown. Injection of pigs with lipopolysaccharide (LPS) can be used to model aspects of poor health. Recent studies have shown that LPS-injected pigs perform
View All Related Papers

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Global Trade Item Number

SKUGTIN
PCYTMAG-23K-0704053252824012
PCYTMAG-23K-1204053252824067
PCYTMAG-23K-0304053252823978
PCYTMAG-23K-1304053252824074
PCYTMAG-23K-10C04055977103397
PCYTMAG-23K-03C04055977103342
PCYTMAG-23K-07C04055977103373
PCYTMAG-23K-06C04055977103366
PCYTMAG-23K-0104053252823954
PCYTMAG-23K-01C04055977107777
PCYTMAG-23K-02C04055977103335
PCYTMAG-23K-04C04055977103359
PCYTMAG-23K-0604053252824005
PCYTMAG-23K-0504053252823992
PCYTMAG-23K-0804053252824029
PCYTMAG-23K-1004053252824043
PCYTMAG-23K-08C04055977103380
PCYTMAG-23K-0904053252824036
PCYTMAG-23K-09C04055977107791
PCYTMAG-23K-0204053252823961
PCYTMAG-23K-0404053252823985
PCYTMAG-23K-13C04055977107807
PCYTMAG-23K-1104053252824050
PCYTMAG-23K-11C04055977103403
PCYTMAG-23K-12C04055977108699

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