usage
sufficient for 100 tests
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
input
sample type intact cells
fluorescence
λex ~485 nm; λem ~535 nm
detection method
fluorometric
storage temp.
−20°C
Quality Level
Biochem/physiol Actions
A broad range of species
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Features and Benefits
Assay Time: 1.5 h
General description
A convenient and sensitive method for the detection of activated caspases in living cells. The fluorescent marker, FITC-VAD-FMK, is a cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspases in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence plate reader, or flow cytometry.
Other Notes
Labeled Caspase Inhibitor (FITC-VAD-FMK), Wash Buffer, Caspase Inhibitor (Z-VAD-FMK), and a user protocol.
Preparation Note
Upon arrival store the entire contents of the kit at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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