ST1070
Anti-Tiam1 Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-T-Cell Lymphoma Invasion and Metastasis 1
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, mouse
Application:
—
Citations:
6
biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting (1:1000))
isotype
IgG
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... TIAM1(7074)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~200 kDa Tiam1 protein.
Recognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.
This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1.
Tiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.
Immunogen
Human
a synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
Application
Immunoblotting (1:1000)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In Tris-Citrate/phosphate, pH 7.0-8.0.
Preparation Note
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Analysis Note
Positive Control
Jurkat or NIH3T3 cells
Jurkat or NIH3T3 cells
Other Notes
Antibody should be titrated for optimal results in individual systems.
Mertens, A.E., et al. 2003. FEBS Lett.546, 11.
Qi, H., et al. 2001. Cell Growth Differ.12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
Qi, H., et al. 2001. Cell Growth Differ.12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Highly Toxic (H)
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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