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Merck

439142

Chloroform

suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Synonym(s):

Methylidyne trichloride, Trichloromethane

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About This Item

Empirical Formula (Hill Notation):
CHCl3
CAS Number:
67-66-3
Molecular Weight:
119.38
PubChem Substance ID:
24867327
UNSPSC Code:
12190000
Beilstein/REAXYS Number:
1731042
MDL number:
MFCD00000826
grade:
HPLC grade
assay:
≥99.8%
technique(s):
HPLC: suitable
application(s):
food and beverages
bp:
60.5-61.5 °C (lit.)
vapor pressure:
160 mmHg ( 20 °C)

Product Name

Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

grade

HPLC grade

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99.8%

form

liquid

contains

0.5-1.0% ethanol as stabilizer

packaging

PVC-coated bottle of 4, 4x4 L

technique(s)

HPLC: suitable

impurities

<0.01% water

evapn. residue

<0.0003%

color

APHA: ≤10

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.48 g/mL at 25 °C
1.492 g/mL at 25 °C (lit.)

cation traces

Pb: ≤0.05 ppm

λ

H2O reference

UV absorption

λ: 245 nm Amax: 1.00
λ: 255 nm Amax: 0.15
λ: 260 nm Amax: 0.15
λ: 270 nm Amax: 0.02
λ: 290-400 nm Amax: 0.01

application(s)

food and beverages

Quality Level

100

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Application

Chloroform was used as an organic phase in the methylene blue (MB) two-phase titration process for estimating the cationic surfactant content in high salinity brine.
It may be used as:
  • Solvent for electropolymerization.
  • Solvent for recrystallization.
  • Extractant in solvent extraction process.

General description

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. It is susceptible to degradation with time, which can be suppressed by adding ethanol as a stabilizer. Since ethanol is added in higher concentration it leads to increase in polarity which may have a potential influence on some of the applications. Reports suggest that chloroform stabilized with ethanol reduces chances on creating artifacts during the chromatographic separation after extraction of anthracyclines from biological matrices.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Packaging

M-Bottle for Solvents
As a global leader in lab reagents, we are constantly looking for new ways to optimize the safety of our products. The newly developed 4L solvent bottle design features advanced sealing technology that eliminates leaks to make the handling of solvents safer and more convenient than ever before.
See all the new features here!

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS7995
SHBS0986
SHBQ1584
SHBQ9222
SHBP4461

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Leyu Cui et al.
Analytical chemistry, 86(22), 11055-11061 (2014-11-05)
The methylene blue (MB) two-phase titration method is a rapid and efficient method for determining the concentrations of anionic surfactants. The point at which the aqueous and chloroform phases appear equally blue is called Epton's end point. However, many inorganic
Solubility controlled electropolymerisation and study of the impact of regioregularity on the spectroelectrochemical properties of thin films of poly (3-octylthiophenes).
Jarosz T, et al.
Electrochimica Acta, 122, 66-71 (2014)
Eagleson M.
Concise Encyclopedia Chemistry, 216-216 (1994)
Traces of phosgene in chloroform: Consequences for extraction of anthracyclines.
Maudens KE, et al.
Journal of Chromatography. B, Biomedical Applications, 848(2), 384-390 (2007)
Electrogenerated chemiluminescent emission from an organized (L-B) monolayer of a Ru(bpy)32+-based surfactant on semiconductor and metal electrodes.
Zhang X and Bard AJ.
The Journal of Physical Chemistry, 92(20), 5566-5569 (1988)
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