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N4638

Neutral Red powder, BioReagent, cell culture | mammalian | 553-24-2

Name: Neutral Red powder, BioReagent, cell culture | mammalian | 553-24-2
Brand: SIAL

BioReagent, Powder

Synonym(s):

Neutral Red, 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red

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About This Item

Empirical Formula (Hill Notation):

C₁₅H₁₇ClN₄

CAS Number:

553-24-2

Molecular Weight:

288.78

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24897707

EC Number:

209-035-8

MDL number:

MFCD00012651

Beilstein/REAXYS Number:

3918740

Colour Index Number:

50040

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Properties

Product Name

Neutral Red powder, BioReagent, cell culture | mammalian | 553-24-2, powder, BioReagent, suitable for cell culture

product line

BioReagent

Quality Segment

200

form

powder

composition

Dye content, ≥90%

technique(s)

cell culture | mammalian: suitable, titration: suitable

color

brown to black

pH

6.8-8.0, red to yellow

mp

290 °C (dec.) (lit.)

solubility

H₂O: 10 mg/mL

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

SMILES string

Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

Description

General description

Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.

Application

Neutral red has been used in several histological staining applications that include:

as a nuclear stain
as a counterstain in the Gram procedure, either alone, or as a component of the Twort stain, a fast green-neutral red mixture.
fluorescent staining of nucleic acids in nervous tissue, of lipids and hydrophobic substances such as suberin and of phenolic compounds in algal cell walls
detection of viable yeast cells in dandruff samples and of mussel larvae
Neutral red has also been used as a stain in flow cytometry.

Useful as an indicator for preparing neutral red paper, and as a biological stain.

Preparation Note

Purified

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c