A1420
Ames′ Medium
With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
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About This Item
NACRES:
NA.75
UNSPSC Code:
41161501
Product Name
Ames′ Medium, With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L
shipped in
ambient
storage temp.
2-8°C
Quality Level
Application
Ames′ Medium has been used to maintain rat/mice/macaque monkey eye for the isolation of retina.
General description
Ames′ Medium has been formulated to support retinal tissue in relatively short-term culture. It is suitable for maintaining central nervous system tissue in vitro.
Preparation Note
Formulated to contain 8.8 grams of powder per liter of medium.
Supplement with 1.9 g/L sodium bicarbonate.
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Frans Vinberg et al.
Vision research, 101, 108-117 (2014-06-25)
The Function of the retina and effects of drugs on it can be assessed by recording transretinal voltage across isolated retina that is perfused with physiological medium. However, building ex vivo ERG apparatus requires substantial amount of time, resources and
Antoine Chaffiol et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(11), 2546-2560 (2017-08-16)
The majority of inherited retinal degenerations converge on the phenotype of photoreceptor cell death. Second- and third-order neurons are spared in these diseases, making it possible to restore retinal light responses using optogenetics. Viral expression of channelrhodopsin in the third-order
Qiuju Jiang et al.
IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society, 26(5), 969-976 (2018-05-13)
Significant progress has been made recently in treating neurological blindness using implantable visual prostheses. However, implantable medical devices are highly invasive and subject to many safety, efficacy, and cost issues. The discovery that ultrasound (US) may be useful as a
B Ameline et al.
Gene therapy, 24(11), 735-741 (2017-09-08)
Several preclinical studies have investigated the potential of algal channelrhodopsin and human melanopsin as optogenetic tools for vision restoration. In the present study, we assessed the potentially deleterious effects of long-term expression of these optogenes on the diseased retina in
John M Nagarah et al.
Frontiers in neuroscience, 9, 384-384 (2015-11-06)
Multielectrode arrays (MEAs) allow for acquisition of multisite electrophysiological activity with submillisecond temporal resolution from neural preparations. The signal to noise ratio from such arrays has recently been improved by substrate perforations that allow negative pressure to be applied to
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