A2237
Phosphatase, Alkaline shrimp
≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host
Synonym(s):
Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
eCl@ss:
42010105
Recombinant:
expressed in proprietary host
Concentration:
≥900 DEA units/mL
recombinant
expressed in proprietary host
Quality Segment
form
buffered aqueous glycerol solution
concentration
≥900 DEA units/mL
technique(s)
protein detection: suitable
foreign activity
RNAse, DNAse, none detected
shipped in
dry ice
storage temp.
−20°C
General description
Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.
Application
Biochem/physiol Actions
Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.
Physical form
solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2
Analysis Note
Protein determined by biuret
Other Notes
One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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