A8185

Ala-Gln

Name: Ala-Gln
Brand: SIGMA

BioReagent, suitable for cell culture, suitable for insect cell culture

Synonym(s):

Alanyl-glutamine, Glutamine-S

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All Photos(3)

About This Item

Empirical Formula (Hill Notation):

C₈H₁₅N₃O₄

CAS Number:

39537-23-0

Molecular Weight:

217.22

UNSPSC Code:

12352209

PubChem Substance ID:

24891211

NACRES:

NA.75

Product Name

Ala-Gln, BioReagent, suitable for cell culture, suitable for insect cell culture

SMILES string

NC(CC[C@H](NC([C@@H](N)C)=O)C(O)=O)=O

InChI

1S/C8H15N3O4/c1-4(9)7(13)11-5(8(14)15)2-3-6(10)12/h4-5H,2-3,9H2,1H3,(H2,10,12)(H,11,13)(H,14,15)/t4-,5-/m0/s1

InChI key

HJCMDXDYPOUFDY-WHFBIAKZSA-N

product line

BioReagent

assay

≥98% (HPLC)

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

color

white to off-white

storage temp.

room temp

Quality Level

300

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Analysis Note

This product is cell culture tested (0.5 mg/ml) and insect cell culture tested (0.2 mg/ml). It is appropriate for use in cell culture and insect cell culture applications at 2-10 mM.

Application

Ala-Gln has been used:

as a supplement in NeuralQ^™ medium
as a component in growth medium and incubation medium (IM) for retinal cell lines
for experimental treatment of retinal cell lines respectively

Biochem/physiol Actions

Alanyl-glutamine (Ala-Gln) is a dipeptide that is used as a substitute for glutamine in mammalian cell culture media, because of the instability of glutamine in solution and the formation of ammonia as a breakdown product of glutamine in solution, which can have deleterious effects on cells in culture. Ala-Glu is stable to heat sterilization and is less ammonia genic than glutamine, which contributes to its advantages as a media component. Ala-Gln has been utilized to investigate polymorphonuclear leucocyte and myeloperoxidase activity in vitro.

Legal Information

NeuralQ is a trademark of GlobalStem

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Effects of supplementation with free glutamine and the dipeptide alanyl-glutamine on parameters of muscle damage and inflammation in rats submitted to prolonged exercise.

Vinicius Fernandes Cruzat et al.

Cell biochemistry and function, 28(1), 24-30 (2009-11-04)

In this study, we investigated the effect of the supplementation with the dipeptide L-alanyl-L-glutamine (DIP) and a solution containing L-glutamine and L-alanine on plasma levels markers of muscle damage and levels of pro-inflammatory cytokines and glutamine metabolism in rats submitted

Effects of oral supplementation with glutamine and alanyl-glutamine on glutamine, glutamate, and glutathione status in trained rats and subjected to long-duration exercise.

Vinicius Fernandes Cruzat et al.

Nutrition (Burbank, Los Angeles County, Calif.), 25(4), 428-435 (2008-12-06)

We investigated the effect of supplementation with the dipeptide L-alanyl-L-glutamine (DIP) and a solution containing L-glutamine and L-alanine, both in the free form, on the plasma and tissue concentrations of glutamine, glutamate, and glutathione (GSH) in rats subjected to long-duration

A high-throughput assay for mRNA silencing in primary cortical neurons in vitro with oligonucleotide therapeutics

Alterman JF, et al.

Bio-protocol, 7(16) (2017)

Growth inhibition in animal cell culture. The effect of lactate and ammonia.

T Hassell et al.

Applied biochemistry and biotechnology, 30(1), 29-41 (1991-07-01)

Eight independent cell lines accumulated ammonia in culture to concentrations between 1.3 and 2.9 mM. The growth inhibition of such concentrations of ammonium chloride when added to culture medium was variable. The cell lines tested could be divided into 3

The adaptation of BHK cells to a non-ammoniagenic glutamate-based culture medium

Christie A, et al.

Biotechnology and Bioengineering, 64(3), 298-309 (1999)

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