Key Documents

View All Documentation

A9647

Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIGMA

heat shock fraction, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

Select a Size

Change View

About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥98%

Form:

lyophilized powder

Technique(s):

immunocytochemistry: suitable

Impurities:

≤5.0% Loss on drying

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

bovine

Quality Level

300

assay

≥98%

form

lyophilized powder

purified by

heat shock fractionation

origin

USA origin

technique(s)

immunocytochemistry: suitable

impurities

≤5.0% Loss on drying

loss

≤5%

pH

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT virus, none detected, VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

General description

Bovine serum albumin is a protein whose key biological function is to regulate the colloidal osmotic pressure of blood. BSA is widely used in various studies and applications. Made up of 583 amino acids, BSA is a water-soluble protein with a calculated molecular weight of 66,430 Da. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Application

Bovine Serum Albumin is used for many applications, including the following applications:

BSA has been used in cell culture, e.g. as a supplement in media prepared for culturing of oligodendrocytes.
BSA has been used for the dilution of primary antibody solution in immunohistochemistry procedures.
BSA has been used as a standard in the Bradford method of protein quantification.
BSA has been used in blocking and permeabilization buffer for immunofluorescence studies.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Still not finding the right product?

Explore all of our products under Bovine Serum Albumin

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Articles

Albumin in Cell Culture

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.