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Merck

B6429

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Synonym(s):

10x Blocking Buffer

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About This Item

NACRES:
NA.54
UNSPSC Code:
12161700

Product Name

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

grade

Molecular Biology

form

solution

technique(s)

Southern blotting: suitable

storage temp.

room temp

Quality Level

200

Related Categories

Application

Casein Blocking Buffer 10x has been used in:

  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

General description

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000500147
0000500147
0000484834
0000484834
0000479651

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Niculescu MD, et al.
Journal of Neurochemistry, 89(5), 1252-1259 (2004)
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(1), 43-49 (2006-01-06)
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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

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