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Merck

C3041

Carbonate-Bicarbonate Buffer

capsule

Synonym(s):

Carbonate Buffer Solution

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

Product Name

Carbonate-Bicarbonate Buffer, capsule

form

capsule

Quality Level

color

colorless

pH

9.3-9.9

solubility

water, high purity: 1 capsule/100 mL

application(s)

diagnostic assay manufacturing

Application

Carbonate-Bicarbonate Buffer has been used for suspending the lyophilized human plasma sample for microtiter plate biofilm assay and flow cell biofilm assay. It has also been used as a coating buffer for microtiter plates prior to enzyme-linked immunosorbent assay(ELISA).
Prepared buffer is suitable for use in protein coating procedures on multiwell plates or plastic tubes.

General description

Bicarbonate buffer mostly have a pH close to 7.4. It is the major small intestine buffer.

Preparation Note

Empty the contents of one capsule in 100 mL of deionized water and dissolve. The contents of one capsule yields 100 ml of 0.05 M carbonate-bicarbonate buffer, pH 9.6 at 25 °C.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Quantifying and comparing constitutive immunity across avian species
Millet S, et al.
Developmental and Comparative Immunology, 31(2), 188-201 (2007)
T J Caperna et al.
Domestic animal endocrinology, 60, 75-82 (2017-05-30)
A simple, reproducible sandwich, ELISA was developed to measure porcine alpha-1 acid glycoprotein (pAGP, ORM-1) in pig plasma. Porcine AGP isolated from serum was purchased and a polyclonal antisera was prepared in rabbits using the whole pAGP molecule as immunogen.
Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)
von Gersdorff J, et al.
Journal of Fish Diseases, 34(10), 749-755 (2011)
Evolution of a physiological pH 6.8 bicarbonate buffer system: application to the dissolution testing of enteric coated products
Liu F, et al.
European Journal of Pharmaceutics and Biopharmaceutics, 78(1), 151-157 (2011)
R M Jaafar et al.
Journal of fish diseases, 41(1), 117-123 (2017-07-15)
Teleosts are able to raise a protective immune response, comprising both innate and adaptive elements, against various pathogens. This is the basis for a widespread use of vaccines, administered as injection or immersion, in the aquaculture industry. It has been

Protocols

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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