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E9762

Endoglycosidase F1

Name: Endoglycosidase F1
Brand: SIGMA

Cleaves within N-linked glycans, suitable for Mass Spectrometry, from Elizabethkingia miricola, recombinant, expressed in E. coli

Synonym(s):

Endo F1, Endo-β-N-acetylglucosaminidase F1, Endoglycosidase F1 from Chryseobacterium meningosepticum, Endoglycosidase F1 from Elizabethkingia meningoseptica, Endoglycosidase F1 from Flavobacterium meningosepticum

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.2.1.96 (BRENDA, IUBMB)

MDL number:

MFCD00146843

Specific activity:

≥16 U/mg

Assay:

≥0.3 mg/mL (Bradford)

Recombinant:

expressed in E. coli

Concentration:

≥0.30 mg/mL

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Properties

Product Name

Endoglycosidase F1 from Elizabethkingia miricola, recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution

recombinant

expressed in E. coli

Quality Segment

200

conjugate

(N-linked)

assay

≥0.3 mg/mL (Bradford)

form

buffered aqueous solution

specific activity

≥16 U/mg

mol wt

32 kDa

concentration

≥0.30 mg/mL

technique(s)

protein mass spectrometry: suitable

optimum pH

5.5

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Substrate Specificity

an N⁴-(oligosaccharide-(1→3)-[oligosaccharide-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] + H₂O = an oligosaccharide-(1→3)-[oligosaccharide-(1→6)]-β-D-Man-(1→4)-D-GlcNAc + N⁴-(N-acetyl-β-D-glucosaminyl)-L-asparaginyl-[protein]

Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.

Application

Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.

Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR) and to glycosylate fucosylated N-glycopeptides.

Biochem/physiol Actions

Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.

Packaging

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄, pH 5.5.

Physical form

Aseptically filled solution in 20 mM Tris-HCl pH 7.5

Other Notes

One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)