G1279

G 418 disulfate salt

Name: G 418 disulfate salt
Brand: SIGMA

powder, suitable for plant cell culture, BioReagent

Synonym(s):

Antibiotic G418

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All Photos(4)

About This Item

Empirical Formula (Hill Notation):

C₂₀H₄₀N₄O₁₀ · 2H₂SO₄

CAS Number:

108321-42-2

Molecular Weight:

692.71

MDL number:

MFCD00058314

UNSPSC Code:

10171502

PubChem Substance ID:

24895058

NACRES:

NA.72

Product Name

G 418 disulfate salt, suitable for plant cell culture, BioReagent

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

product line

BioReagent

form

powder

potency

≥720 μg per mg (Dried basis)

technique(s)

cell culture | plant: suitable

color

white to off-white

solubility

100 mg/mL (H₂O clear)

antibiotic activity spectrum

Gram-negative bacteria

application(s)

agriculture

mode of action

protein synthesis | interferes

storage temp.

2-8°C

Quality Level

200

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Related Categories

Antibiotics

Biochemicals

Cell Culture & Analysis

Cell Culture Media & Buffers

Cell Culture Supplements & Reagents

Disclaimer

The product is stable for three years when stored at 2-8°C.

General description

Chemical structure: aminoglycoside

Preparation Note

G418 powder is soluble in water at 100 mg/mL.

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000508844

0000453338

0000453339

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Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients.

Frida C Mohlin et al.

Molecular immunology, 65(2), 367-376 (2015-03-04)

Atypical hemolytic uremic syndrome (aHUS) is a disease of complement dysregulation, characterized by hemolytic anemia, thrombocytopenia and acute renal failure. Mutations in complement inhibitors are major risk factors for development of aHUS. The three aHUS patients reported in this study

Anti-MPER antibodies with heterogeneous neutralization capacity are detectable in most untreated HIV-1 infected individuals.

Luis M Molinos-Albert et al.

Retrovirology, 11, 44-44 (2014-06-10)

The MPER region of the HIV-1 envelope glycoprotein gp41 is targeted by broadly neutralizing antibodies. However, the localization of this epitope in a hydrophobic environment seems to hamper the elicitation of these antibodies in HIV infected individuals.We have quantified and

Modeling retinitis pigmentosa through patient-derived retinal organoids.

Yan-Ping Li et al.

STAR protocols, 2(2), 100438-100438 (2021-04-27)

Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR)

NR4A1 promotes PDGF-BB-induced cell colony formation in soft agar.

Glenda Eger et al.

PloS one, 9(9), e109047-e109047 (2014-10-01)

The fibroblast mitogen platelet-derived growth factor -BB (PDGF-BB) induces a transient expression of the orphan nuclear receptor NR4A1 (also named Nur77, TR3 or NGFIB). The aim of the present study was to investigate the pathways through which NR4A1 is induced

Identities of P2 and P3 Residues of H-2Kb-Bound Peptides Determine Mouse Ly49C Recognition.

Elsa A Marquez et al.

PloS one, 10(7), e0131308-e0131308 (2015-07-07)

Ly49 receptors can be peptide selective in their recognition of MHC-I-peptide complexes, affording them a level of discrimination beyond detecting the presence or absence of specific MHC-I allele products. Despite this ability, little is understood regarding the properties that enable

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