G7663

Gelatin blocking buffer

Name: Gelatin blocking buffer
Brand: SIGMA

for Western blotting, powder blend

Synonym(s):

blocking buffer

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About This Item

NACRES:

NA.25

UNSPSC Code:

41105300

Product Name

Gelatin blocking buffer, for Western blotting, powder blend

grade

Molecular Biology

form

powder blend

storage temp.

room temp

Quality Level

100

Related Categories

Immunodetection Reagents & Substrates

Protein Biology

Protein Electrophoresis & Western Blotting

Application

Suitable for use as a non-specific blocking agent for Western blots.

General description

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Preparation Note

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.