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I3769

Insulin-like Growth Factor-I human

Name: Insulin-like Growth Factor-I human
Brand: SIGMA

≥95% (HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IGF, IGF-I, somatomedin c

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00081716

Biological source:

human

Recombinant:

expressed in E. coli

Assay:

≥95% (HPLC)

Form:

lyophilized powder

Mol wt:

~7.6 kDa

Impurities:

≤1.00 EU/μg

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Properties

Product Name

Insulin-like Growth Factor-I human, IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in E. coli

assay

≥95% (HPLC)

form

lyophilized powder

potency

0.1-10 ng/mL ED₅₀/EC₅₀

quality

endotoxin tested

mol wt

~7.6 kDa

packaging

pkg of 50 and 100 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg

UniProt accession no.

P05019

storage temp.

−20°C

Gene Information

human ... IGF1(3479), Igf1(16000)

Description

General description

Insulin-like growth factor I (IGF-I) is localized on human chromosome 12q23.2.

Application

Insulin-like Growth Factor-I human has been used:

in modified Dulbecco minimal essential medium for in vitro culture of testicular cells
in culture media, to study its effect on steroidogenesis during testicular development
in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation

Biochem/physiol Actions

Insulin-like growth factor I (IGF-I) plays a critical role in cellular energy metabolism and growth and development, especially prenatal growth.

IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.

IGF-I, also known as somatomedin C, mediates the growth-promoting activity of GH (growth hormone). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis. IGF-I exerts its actions through the IGF-I receptor.

Physical form

Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid.

Preparation Note

Reconstitute in water

Analysis Note

The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED₅₀ corresponds to a specific activity of at least 1 x 10⁵ units/mg.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c