L0144

Lucifer Yellow CH dipotassium salt

Name: Lucifer Yellow CH dipotassium salt
Brand: SIGMA

Intracellular staining dye, powder

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About This Item

Empirical Formula (Hill Notation):

C₁₃H₉K₂N₅O₉S₂

CAS Number:

71206-95-6

Molecular Weight:

521.57

NACRES:

NA.77

PubChem Substance ID:

24896249

UNSPSC Code:

12171500

MDL number:

MFCD00013445

Beilstein/REAXYS Number:

5224942

Product Name

Lucifer Yellow CH dipotassium salt, powder

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

form

powder

color

orange

fluorescence

λ_ex 428 nm; λ_em 540 nm

storage temp.

room temp

Quality Level

100

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Related Categories

Bioactive Small Molecules

Application

Lucifer yellow CH dipotassium salt has been used:

to visualize cell morphology
to assess intercellular communication activity through gap junction by scrape-loading assay
as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology
in the intracellular solution, to intracellularly label recorded neurons

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.LuciferYellow CH (LYCH) is a small, hydrophilic molecule, and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells. It acts as a marker for the detection of well-established tight junctions in smooth muscles, glia, and interstitial cells of Cajal (ICC).

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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In vitro mesenchymal-epithelial transition in NIH3T3 fibroblasts results in onset of low-dose radiation hypersensitivity coupled with attenuated connexin-43 response

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Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.

Shuaiwei Wang et al.

Mediators of inflammation, 2020, 8094347-8094347 (2020-12-10)

Connexin (Cx) family members form hemichannels (HCs) and gap junctions (GJs). Biological functions of Cx HCs have not been adequately characterized due to the inability to selectively target HCs or GJs. Recently, we developed a 6-mer peptide mimetic (P5) of

Zebrafish retinal slice preparation

Connaughton, VP

Methods in Cell Science : An Official Journal of the Society for In Vitro Biology, 25(1-2), 49-58 (2003)

Connexin-43 downregulation in G2/M phase enriched tumour cells causes extensive low-dose hyper-radiosensitivity (HRS) associated with mitochondrial apoptotic events

Ghosh S, et al.

Cancer letters, 363(1), 46-59 (2015)

In vitro and in vivo models of BBB to evaluate brain targeting drug delivery

Sharma B, et al.

Brain, 53-101 (2019)

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