L4402

Lysostaphin from Staphylococcus staphylolyticus

Name: Lysostaphin from <I>Staphylococcus staphylolyticus</I>
Brand: SIGMA

BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

Synonym(s):

Glycyl-glycine Endopeptidase

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All Photos(6)

About This Item

CAS Number:

9011-93-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.24.75 (BRENDA, IUBMB)

MDL number:

MFCD00131556

Product Name

Lysostaphin from Staphylococcus staphylolyticus, BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

biological source

bacterial (Staphylococcus sp.)

product line

BioUltra

assay

≥97% (SDS-PAGE)

form

lyophilized powder

specific activity

≥2,000 units/mg protein

mol wt

25 kDa

composition

Protein, 40-60% biuret

concentration

40—60% protein

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

antibiotic activity spectrum

Gram-positive bacteria

application(s)

diagnostic assay manufacturing

mode of action

cell wall synthesis | interferes

storage temp.

−20°C

Quality Level

200
300

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Related Categories

Biochemicals

High-Purity Biochemicals and Reagents

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

General description

Chemical structure: peptide

Package size based on protein content

Other Notes

One unit will reduce the turbidity (A₆₂₀) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

Physical form

Contains potassium phosphate buffer salts and sodium chloride

Preparation Note

Affinity purified

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P304 + P340 + P312 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Sustained Circulating Bacterial Deoxyribonucleic Acid Is Associated With Complicated Staphylococcus aureus Bacteremia.

Johnny Gutierrez et al.

Open forum infectious diseases, 6(4), ofz090-ofz090 (2019-04-27)

Staphylococcus aureus (SA) bacteremia often requires a long treatment duration with antibiotics to prevent relapse due to the ability of SA to establish reservoirs of infection in sites such as heart and bone. These metastatic sites of infection cannot be

Optimization of a two-step permeabilization fluorescence in situ hybridization (FISH) assay for the detection of Staphylococcus aureus.

Thomas S Lawson et al.

Journal of clinical laboratory analysis, 25(5), 359-365 (2011-09-16)

Aspects of the fluorescence in situ hybridization (FISH) method for the detection of clinically important bacteria, such as Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli, were investigated for optimization. Various approaches to optimizing the FISH procedure were taken and different

Antibody-directed targeting of lysostaphin adsorbed onto polylactide nanoparticles increases its antimicrobial activity against S. aureus in vitro.

R Satishkumar et al.

Nanotechnology, 22(50), 505103-505103 (2011-11-24)

The objective of this paper was to study the effect of antibody-directed targeting of S. aureus by comparing the activities of lysostaphin conjugated to biodegradable polylactide nanoparticles (NPs) in the presence and in the absence of co-immobilized anti-S. aureus antibody.

Inhibition of the activity of both domains of lysostaphin through peptidoglycan modification by the lysostaphin immunity protein.

Shaw R Gargis et al.

Applied and environmental microbiology, 76(20), 6944-6946 (2010-08-24)

Resistance to lysostaphin, a staphylolytic glycylglycine endopeptidase, is due to a FemABX-like immunity protein that inserts serines in place of some glycines in peptidoglycan cross bridges. These modifications inhibit both binding of the recombinant cell wall targeting domain and catalysis

Antibacterial mesh: a novel technique involving naturally occurring cellular proteins.

Yuliya Yurko et al.

Surgical innovation, 19(1), 20-26 (2011-07-12)

Naturally occurring antimicrobial peptides are possibly the "next frontier" in infection prevention. Binding them to mesh could reduce the rate of mesh infections. This study identifies an antimicrobial agent capable of significant antibacterial activity when bound to mesh. Lysozyme, human

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