L5263
Lyticase from Arthrobacter luteus
partially purified powder, ≥1,500 units/mg protein
Product Name
Lyticase from Arthrobacter luteus, partially purified powder, ≥1,500 units/mg protein
form
partially purified powder
specific activity
≥1,500 units/mg protein
composition
Protein, ≥10% biuret
storage temp.
2-8°C
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Other Notes
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Physical form
Partially purified powder containing ammonium sulfate and stabilizer
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M L Branchini et al.
Journal of clinical microbiology, 32(2), 452-456 (1994-02-01)
Candida parapsilosis is an important nosocomial pathogen that can proliferate in high concentrations of glucose and form biofilms on prosthetic materials. We investigated the genotypic diversity and slime production among 31 isolates of C. parapsilosis from individual patients with bloodstream
Markus M M Bisschops et al.
Biochimica et biophysica acta, 1864(1), 231-242 (2016-11-08)
Non-dividing Saccharomyces cerevisiae cultures are highly relevant for fundamental and applied studies. However, cultivation conditions in which non-dividing cells retain substantial metabolic activity are lacking. Unlike stationary-phase (SP) batch cultures, the current experimental paradigm for non-dividing yeast cultures, cultivation under
B N Doebbeling et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 16(3), 377-383 (1993-03-01)
Candida species are important nosocomial pathogens, particularly in immunocompromised and critically ill patients. A variety of methods have been used to differentiate strains, but an optimal system has not been established. We compared methods for typing a panel of nine
Construction of chromosome-specific libraries of yeast artificial chromosome recombinants from somatic hybrid cell lines.
B Arveiler
Methods in molecular biology (Clifton, N.J.), 29, 379-402 (1994-01-01)
Sanford J Silverman et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(15), 6946-6951 (2010-03-26)
Oscillations in patterns of expression of a large fraction of yeast genes are associated with the "metabolic cycle," usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that
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