P2192
10X PCR Buffer
Optimized for routine PCR with MgCl2 included
Synonym(s):
PCR Buffer Solution, PCR Reaction Buffer
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About This Item
NACRES:
NA.52
UNSPSC Code:
41106306
Quality Level
form
liquid
packaging
vial of 1.5 mL
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
storage temp.
−20°C
Application
10X PCR Buffer has been used as a component of the PCR mixture:
- for the amplification of viral DNA
- to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
- to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.
Features and Benefits
- Standalone buffer
- Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)
Preparation Note
Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Protocols
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
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