S6639
SSC Buffer 20× Concentrate
for Northern and Southern blotting, solution
Synonym(s):
20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer
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About This Item
grade
Molecular Biology
Quality Level
description
Saline-Sodium Citrate buffer, made with ultrapure water
sterility
0.2 μm filtered
form
solution
color
colorless
pH
6.9 —7.1
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
Application
- as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
- as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
- as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
Preparation Note
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Articles
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.