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23-022-M

UbcH3 Protein, active, 10 µg

Active, full length, recombinant human UbcH3. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

Synonym(s):

Ubiquitin-conjugating enzyme E2 R1, E2-CDC34, UBE2R1, CDC34, Ubiquitin-protein ligase R1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
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biological source

human

Quality Segment

100

recombinant

expressed in baculovirus infected Sf21 cells

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

NCBI accession no.

NM_004359

General description

Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or an Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate.
UbcH3 has been shown to interact with a number of different E3 ligases, including SCFSkp2/Cks1, promoting the ubiquitination of p27 and SCFβTrCP1, promoting the ubiquitination of β-catenin and IκBα.

Application

Active, full length, recombinant human UbcH3. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Related Content

Detecting Substrate Ubiquitination by the E3 Ligase, XIAP, Using Quantitative Assays

Quantitative assays for investigating ubiquitination pathways are essential for advancing ubiquitin-related drug discovery research. To illustrate the utility of in vitro ubiquitination enzyme cascades, we quantify the ubiquitination of Smac/DIABLO by the E3 ligase XIAP using a functional enzymatic reaction coupled to multiple assay readouts. We compare different detection methods that can be used to test small molecules for their potential to modulate XIAP-mediated ubiquitination in the context of this assay. The data provides a basis for use of these recombinant E3 ligase cascades in various applications, including screening campaigns.

Global Trade Item Number

SKUGTIN
23-02204053252294679
23-022M04053252294389