08124
Isoamylase from Pseudomonas sp.
ammonium sulfate suspension, ≥10,000,000 units/mg protein
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About This Item
Specific activity:
≥10,000,000 units/mg protein
form
ammonium sulfate suspension
Quality Level
specific activity
≥10,000,000 units/mg protein
storage temp.
2-8°C
Application
Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.
Biochem/physiol Actions
Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.
Analysis Note
≥10 MU/mg protein
Other Notes
One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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In vitro amylase hydrolysis of amylopectins from cereal starches based on molecular structure of amylopectins
Guo Li
Food Hydrocolloids, 77, 238-247 (2018)
Digestion properties of corn starch modified by $\alpha$-D-glucan branching enzyme and cyclodextrin glycosyltransferase
Li Y, et al.
Food Hydrocolloids, 89, 534-541 (2019)
Wenwen Li et al.
Carbohydrate polymers, 270, 118363-118363 (2021-08-09)
Finding an efficient and eco-friendly solution for starch dissolution has attracted considerable attentions in recent years. This study investigated the structural characteristics, and degradation behavior of corn starch in KOH/thiourea aqueous solution by the comparison with DMSO/LiBr and 1-allyl-3-methylimidazolium chloride