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H9773

ハイグロマイシンB Streptomyces hygroscopicus由来

Name: ハイグロマイシンB <I>Streptomyces hygroscopicus</I>由来
Brand: SIGMA

lyophilized powder, suitable for plant cell culture, BioReagent

別名:

Hygromycin

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この商品について

実験式（ヒル表記法）:

C₂₀H₃₇N₃O₁₃

CAS番号:

31282-04-9

分子量:

527.52

NACRES:

NA.72

PubChem Substance ID:

329815270

UNSPSC Code:

12352207

EC Number:

250-545-5

MDL number:

MFCD06795479

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特性

製品名

ハイグロマイシンB Streptomyces hygroscopicus由来, suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder

biological source

Streptomyces hygroscopicus

Quality Segment

200

product line

BioReagent

form

lyophilized powder

purified by

ion-exchange chromatography

concentration

≥60% (HPLC)

technique(s)

cell culture | plant: suitable

color

faintly brown to brown, white to beige

(1) 7.1, (2) 8.8

solubility

H₂O: soluble 50 mg/mL, ethanol: soluble, methanol: soluble

antibiotic activity spectrum

fungi

application(s)

agriculture

mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

詳細

General description

Chemical structure: aminoglycoside

Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

Application

ヒグロマイシンBは、分子生物学的研究において、ヒグロマイシンホスホトランスフェラーゼ(hpt)などの選択的マーカー遺伝子により形質転換される植物細胞の選択剤として用いられます。

Biochem/physiol Actions

作用機序：本製品は、原核生物におけるm-RNAテンプレートの誤読を誘発してタンパク質合成を阻害することにより作用し、翻訳を阻害する効力があります。

抗菌スペクトル：ヒグロマイシンBは、細菌、真菌およびより高等な真核細胞に対して作用します。

Hygromycin B is used in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. It is also adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans. It can be inactivated by Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) through phosphorylation. The amino function of this antibiotic may be involved in a general acid-base catalysis performed by the ribozyme, acting as proton acceptors/donors.

Preparation Note

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

Disclaimer

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.

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pictograms

GHS06

signalword

Danger

hcodes

H300 + H310 + H330

pcodes

P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

保管分類

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c