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A4284

L-Asparagine monohydrate

Name: <SC>L</SC>-Asparagine monohydrate
Brand: SIGMA

BioReagent, suitable for cell culture

Synonym(s):

(S)-(+)-2-Aminosuccinamic acid, (S)-2-Aminosuccinic acid 4-amide, L-Aspartic acid 4-amide

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About This Item

Linear Formula:

NH₂COCH₂CH(NH₂)COOH · H₂O

CAS Number:

5794-13-8

Molecular Weight:

150.13

UNSPSC Code:

12352209

NACRES:

NA.75

PubChem Substance ID:

24890843

EC Number:

200-735-9

Beilstein/REAXYS Number:

1723527

MDL number:

MFCD00151038

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Properties

biological source

synthetic

Quality Level

300

product line

BioReagent

assay

≥99% (TLC)

form

powder

technique(s)

cell culture | mammalian: suitable

color

white to off-white

mp

233-235 °C (lit.)

SMILES string

[H]O[H].N[C@@H](CC(N)=O)C(O)=O

InChI

1S/C4H8N2O3.H2O/c5-2(4(8)9)1-3(6)7;/h2H,1,5H2,(H2,6,7)(H,8,9);1H2/t2-;/m0./s1

InChI key

RBMGJIZCEWRQES-DKWTVANSSA-N

Description

Application

L-Asparagine monohydrate has been used:

as a media supplement to culture Black Mexican Sweet (BMS) cells
to prepare Adams III media to supplement C+Y medium for culturing Streptococcus pneumoniae
in an asparagine-rich diet to study the E0771 syngeneic breast tumor growth and survival in mice

Biochem/physiol Actions

L-Asparagine (L-Asp) is a nonessential amino acid involved in the synthesis of critical cellular proteins of human cells. It is synthesized from oxaloacetic acid. L-Asp is used as a growth supplement for tumor cells in in vitro conditions.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.