B0174
AccuTaq™ LA 10× Buffer
10X Buffer for long and accurate PCR
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About This Item
UNSPSC Code:
41106306
grade
Molecular Biology
Quality Level
form
liquid
packaging
vial of 0.5 mL
concentration
10 ×
technique(s)
PCR: suitable
storage temp.
−20°C
General description
AccuTaq™ LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase.
Application
AccuTaq™ LA 10× Buffer has been used:
- in PCR reaction mixture for the amplification of human BRCA1 gene.
- as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum
- as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV)
Legal Information
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
Hameer Ruparel et al.
Genome research, 14(2), 296-300 (2004-02-06)
Deletion or insertion mutations lead to a frameshift that causes misalignment between wild-type and mutated allele sequences, making it difficult to identify such mutations unambiguously by using electrophoresis-based DNA sequencing. We have previously established the feasibility of an accurate DNA
Eva-Maria Ladenburger et al.
Molecular and cellular biology, 29(13), 3605-3622 (2009-04-22)
A database search of the Paramecium genome reveals 34 genes related to Ca(2+)-release channels of the inositol-1,4,5-trisphosphate (IP(3)) or ryanodine receptor type (IP(3)R, RyR). Phylogenetic analyses show that these Ca(2+) release channels (CRCs) can be subdivided into six groups (Paramecium
Jeong-Yun Choi et al.
The Journal of biological chemistry, 286(36), 31180-31193 (2011-07-26)
The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts. Both mRNAs and proteins of all four DNA