Key Documents

View All Documentation

108975

Leupeptin, Hemisulfate, Microbial

Name: Leupeptin, Hemisulfate, Microbial
Brand: MM

Leupeptin hemisulfate, CAS 103476-89-7, is a reversible inhibitor of trypsin-like proteases and cysteine proteases

Synonym(s):

Leupeptin, Hemisulfate, Microbial, Ac-LLR-CHO, ½H₂SO₄

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₂₀H₃₈N₆O₄ · 0.5H₂SO₄

CAS Number:

103476-89-7

Molecular Weight:

475.59

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00037012

Assay:

≥90% (HPLC)

Form:

lyophilized solid

Quality level:

200

Storage condition:

OK to freeze, desiccated (hygroscopic)

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Quality Level

200

description

Merck USA index - 14, 5456

assay

≥90% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic)

color

white

solubility

water: 50 mg/mL

shipped in

ambient

storage temp.

−20°C

SMILES string

[N+H](=C(N)N)CCC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)CC(C)C)CC(C)C)C=O

InChI

1S/C20H38N6O4/c1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22/h11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23)/p+1/t15-,16-,17-/m0/s1

InChI key

GDBQQVLCIARPGH-ULQDDVLXSA-O

Description

General description

A reversible inhibitor of trypsin-like proteases and cysteine proteases. Also known to inhibit activation-induced programmed cell death and to restore defective immune response in HIV⁺ donors. Often used for the inhibition of endoproteinase Lys-C, kallikrein, papain, plasmin, thrombin, cathepsin B, and trypsin. Leupeptin does not inhibit chymotrypsin, elastase, renin, or pepsin. The recommended working range is 10-100 µM for most applications.

Biochem/physiol Actions

Cell permeable: no

Primary Target
trypsin- and cysteine-like proteases

Product does not compete with ATP.

Reversible: yes

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Aqueous stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C.

Other Notes

Ac-Leu-Leu-arginal, hemisulfate

Montenez, J.P., et al. 1994. Toxicol. Lett. 73, 201.
Sarin, A., et al. 1994. J. Immunol.153, 862.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c