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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA, IHC, WB
Citations:
112
biological source
rabbit
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
goat
manufacturer/tradename
Chemicon®
concentration
1 mg/mL (after reconstitution)
technique(s)
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
General description
Affinity purified rabbit anti-goat IgG, Peroxidase conjugated.
Application
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
1:5,000-1:100,000 dilution can be used.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
Rabbit anti-Goat IgG Antibody, HRP conjugate is an antibody against Goat IgG for use in ELISA, IH & WB.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
Biochem/physiol Actions
Specific for goat IgG, heavy and light chain.
Physical form
Immunoaffinity chromatography using antigen coupled to sepharose beads.
Purified Rabbit IgG conjugated to horseradish peroxidase in buffer containing 0.01M PBS, pH 7.1, with 15mg/mL BSA. Lyophilized.
Preparation Note
Lyophilized product is stable at 2-8°C for up to 12 months from date of shipment.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
RABBIT ANTI GOAT IgG (H & L) HORSERADISH PEROXIDASE CONJUGATED
The influence of pKA treatment on the ca2+ activation of force generation by trout cardiac Muscle.
Gillis TE, Klaiman JM
The Journal of Experimental Biology null
Rebeca López-Úbeda et al.
PloS one, 10(6), e0130128-e0130128 (2015-06-23)
Gene Expression Microarray technology was used to compare oviduct transcriptome between inseminated and non-inseminated pigs during spontaneous oestrus. We used an in vivo model approaching the study from a physiological point of view in which no hormonal treatment (animals were
The influence of trout cardiac troponin I and PKA phosphorylation on the Ca2+ affinity of the cardiac troponin complex.
Kirkpatrick KP, Robertson AS, Klaiman JM, Gillis TE.
The Journal of Experimental Biology null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AP106P | 04053252582851 |