43925
Nigrosin Stain solution
suitable for microscopy
form
liquid
shelf life
limited shelf life, expiry date on the label
technique(s)
microbe id | staining: suitable
suitability
suitable for microscopy, Bacillus spp., Corynebacterium spp.
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
General description
Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans.
Other Notes
Composition:
Nigrosin 100 g/l, Formalin 5 ml/l in water
Nigrosin 100 g/l, Formalin 5 ml/l in water
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves
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G E Freneau et al.
Animal reproduction science, 118(2-4), 176-181 (2009-09-30)
The objectives of this study were to compare two different methods of evaluating bull sperm morphology, bright-field (BF) microscopy of eosin-nigrosin (EN) stained dry-mount semen smears and differential interference phase contrast (DIC) microscopy of wet-mount semen 'fixed' in isotonic formal
Jie Guo et al.
Environmental science & technology, 42(2), 624-628 (2008-02-21)
Nonmetals reclaimed from waste phenolic cellulose paper printed circuit boards (PCBs) are used to replace wood flour in the production of phenolic molding compound (PMC). The results indicate that filling of nonmetals in PMC improves the charpy notched impact strength
D Kutter et al.
Clinica chimica acta; international journal of clinical chemistry, 258(2), 231-239 (1997-02-17)
Nigrosin binds to proteins yielding a dark blue complex. This reaction is used for detecting low protein concentrations in urine by applying spots of urine to cellulose acetate strips which are coloured by nigrosin. The intensity of coloured spots remaining
J Moshonov et al.
Endodontics & dental traumatology, 11(5), 220-224 (1995-10-01)
The purpose of this study was to assess the efficacy of Nd:YAG laser irradiation in disinfecting the root canal system. Seventy-five teeth were uniformly instrumented and sterilized, and their root canals were infected for 60 min with an overnight culture
K Matsuda et al.
Journal of clinical laboratory analysis, 15(4), 171-174 (2001-07-04)
We designed a semiquantitative analysis of urinary low protein levels using silver dot blot assay. In this method, 3 microl of urine are blotted to one dot onto a cellulose acetate membrane, which is stained by a colloidal silver staining
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