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Merck

A8251

Acriflavine hydrochloride

Powder

Synonym(s):

3,6-Diamino-10-methylacridinium chloride hydrochloride, Euflavine

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About This Item

CAS Number:
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
6265525
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Product Name

Acriflavine hydrochloride, fluorescent dye

InChI

1S/C14H13N3.C13H11N3.3ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;;;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;3*1H

SMILES string

[Cl-].Cl[H].Cl[H].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]4c5cc(N)ccc5cc6ccc(N)cc46

InChI key

MKLTXAHQKDVBLY-UHFFFAOYSA-N

form

powder

composition

Acriflavine HCL content, 10-50%
Proflavine HCL, 25-70%

technique(s)

microbe id | staining: suitable

color

faint brown to brown, and Orange to Dark Orange and Red to Very Dark Red

mp

260 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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Application

In living mice, acriflavine hydrochloride has been used in the confocal laser endomicroscopy to check the crypt structure in mucosa. It has also been used in Neurospora strain selection by checking the acriflavine resistance.

Biochem/physiol Actions

Acriflavine hydrochloride is a fluorescent dye. It is used as a topical contrast agent. It mainly stains nuclei and is applied to tissues before microscopy.

Other Notes

A mixture of 3,6-diamino-10-methylacridinium (acriflavin) and 3,6-diaminoacridine (proflavin)

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Xiangnan Du et al.
Frontiers in aging neuroscience, 12, 54-54 (2020-03-27)
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period-1 encodes an ATP-dependent RNA helicase that influences nutritional compensation of the Neurospora circadian clock.
Emerson JM, et al.
Proceedings of the National Academy of Sciences of the USA, 112, 15707-15712 (2015)
G Duarte et al.
International journal of food microbiology, 52(3), 163-168 (2000-03-25)
Four secondary enrichment protocols (conventional methods: UVM II, Fraser 24 h and Fraser 48 h: Impedimetric method: Listeria electrical detection medium) were studied for their ability to isolate Listeria spp. and Listeria monocytogenes from fish and environmental samples collected along
Nashwa Amin et al.
Cells, 10(6) (2021-07-03)
Hypoxia-inducible factor 1 can sufficiently control the progress of neurological symptoms after ischemic stroke owing to their actions associated with its downstream genes. In this study, we evaluated the role of HIF-1α in attenuating brain damage after endothelin-1 injection. Focal
Yang Hua et al.
Journal of AOAC International, 95(6), 1672-1688 (2013-03-05)
The Roka Listeria Detection Assay was compared to the reference culture methods for nine select foods and three select surfaces. The Roka method used Half-Fraser Broth for enrichment at 35 +/- 2 degrees C for 24-28 h. Comparison of Roka's

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