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Merck

GERPN1236

Anti-GST HRP Conjugate

Cytiva RPN1236, pack of 75 μL

Synonym(s):

ECL, detection reagent, label, probe, secondary antibody

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203

Product Name

Anti-GST HRP Conjugate, Cytiva RPN1236, pack of 75 μL

conjugate

peroxidase conjugate

antibody product type

primary antibodies

packaging

pack of 75 μL

manufacturer/tradename

Cytiva RPN1236

color

Colorless

shipped in

dry ice

storage temp.

−20°C

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Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Legal Information

ECL is a trademark of Cytiva
GST Gene Fusion Vectors
A license for commercial use of GST Gene Fusion Vectors under US patent 5,654,176 and equivalent patents and patent applications in other countries must be obtained from Millipore Corp (formerly Chemicon International Inc).

General description

Anti-GST HRP Conjugate.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion Proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL Detection Reagents.
GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion Proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL Detection Reagents.

Features and Benefits

  • Offers the advantages of speed, sensitivity, increased safety, and convenience when used with ECL detection systems. Application tested with ECL and ECL Plus reagents in western blotting for sensitive hard copy results
  • Detection of GST is simplified because no secondary antibody is required
  • The conjugate recognizes multiple epitopes of GST and therefore is not reliant on functional GST for fusion Protein detection. It has also been cross-absorbed to E. coli Proteins so that GST-fusion Proteins can be easily and specifically identified against an E. coli background.
  • Binds with high affinity to GST fusion Proteins.
  • Also suitable for colorimetric detection with diaminobenzidine (DAB) and tetramethylbenzidine (TMB).
  • ECL optimized protocols included.

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Storage Class

12 - Non Combustible Liquids


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Hannes Vanhaeren et al.
eLife, 9 (2020-03-27)
Protein ubiquitination is a very diverse post-translational modification leading to protein degradation or delocalization, or altering protein activity. In Arabidopsis thaliana, two E3 ligases, BIG BROTHER (BB) and DA2, activate the latent peptidases DA1, DAR1 and DAR2 by mono-ubiquitination at
Asparagine endopeptidase cleaves α-synuclein and mediates pathologic activities in Parkinson's disease.
Zhang Z, et al.
Nature Structural and Molecular Biology, 24(8), 632-632 (2017)
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Nature communications, 9(1), 5016-5016 (2018-11-28)
The repair of double-stranded DNA breaks (DSBs) by homologous recombination involves the formation of branched intermediates that can lead to crossovers following nucleolytic resolution. The nucleases Mus81-Mms4 and Yen1 are tightly controlled during the cell cycle to limit the extent
Zhentao Zhang et al.
Nature communications, 8, 14740-14740 (2017-03-28)
δ-secretase, also known as asparagine endopeptidase (AEP) or legumain, is a lysosomal cysteine protease that cleaves both amyloid precursor protein (APP) and tau, mediating the amyloid-β and tau pathology in Alzheimer's disease (AD). Here we report the therapeutic effect of
Amra Saric et al.
Nature communications, 12(1), 4552-4552 (2021-07-29)
The ability of endolysosomal organelles to move within the cytoplasm is essential for the performance of their functions. Long-range movement involves coupling of the endolysosomes to motor proteins that carry them along microtubule tracks. This movement is influenced by interactions

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