L4895
Lectin from Triticum vulgaris (wheat)
FITC conjugate, lyophilized powder
Synonym(s):
WGA, Wheat germ agglutinin
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Product Name
Lectin from Triticum vulgaris (wheat), FITC conjugate, lyophilized powder
conjugate
FITC conjugate
form
lyophilized powder
potency
<50 μg/mL agglutination activity
composition
Protein, ~50% Lowry
extent of labeling
~2 mol FITC per mol protein
storage temp.
2-8°C
Quality Level
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Related Categories
Application
For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Lectin from Triticum vulgaris (wheat) has been used:
- to treat transfected cells for immunofluorescence
- to stereotaxically inject into mice for neuronal tracing
- in immunohistochemistry for the analysis of myocytes
Biochem/physiol Actions
Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
General description
Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.
Packaging
Package size based on protein content.
Physical form
Contains phosphate buffer salts
Preparation Note
Conjugates are prepared from affinity purified lectin.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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