Anti-Acetylated Tubulin antibody, Mouse monoclonal

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Monoclonal anti-acetylated tubulin antibody (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 50,000 and share considerable homology.

acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in:

• quantitative dot blot
• immunofluorescence
• Western blot
• immunocytochemistry
• enzyme-linked immunosorbent assay (ELISA)
• solid-phase radioimmunoassay (RIA)
• electron microscopy

Anti-acetylated tubulin antibody has also been used in staining X-gal–stained paraffin sections to perform immunohistochemistry.

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys⁴⁰ when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corre­sponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK₂.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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