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Merck

203389

Blebbistatin, Racemic

≥97% (HPLC), liquid, Myosin II inhibitor, Calbiochem®

Synonym(s):

InSolutionBlebbistatin, Racemic

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About This Item

Empirical Formula (Hill Notation):
C18H16N2O2
Molecular Weight:
292.33
UNSPSC Code:
12352200
NACRES:
NA.77
Assay:
≥97% (HPLC)
Form:
liquid
Quality level:
100
Storage condition:
OK to freeze
desiccated (hygroscopic)
protect from light

Key Documents

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Product Name

Blebbistatin, Racemic, InSolution, ≥97%, 50 mM in 90% DMSO, reversible inhibitor of nonmuscle myosin II

assay

≥97% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: yes
Primary Target
ATPase
Product does not compete with ATP.
Reversible: yes
Target IC50: <100 µM inhibiting the ATPase and gliding motility of human platelets

Disclaimer

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

General description

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of non-muscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly.

Other Notes

Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.

Packaging

Packaged under inert gas

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203390 ) in 90% DMSO.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F - closed cup - (Dimethylsulfoxide)

flash_point_c

87 °C - closed cup - (Dimethylsulfoxide)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Group 4: Flammable liquids + Type 3 petroleums + Hazardous rank III + Water insoluble liquid

fsl

203389-5MG:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
203389-5MGCN04055977221251

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