J14 Human SLP-76-Deficient Jurkat Cell Line

J14 cells are a unique Jurkat-derived human T cell line that lacks expression of SLP-76 (SH2 domain–containing leukocyte protein of 76 kilodaltons), an adaptor protein and PTK substrate expressed in hematopoietic cells (2). SLP-76 together with the scaffolding protein LAT act as a hub for various signaling effectors (1). Except SLP-76, the expression profile of J14 cells encompasses all necessary components of downstream pathways including those required for IL-2 cytokine induction (3), making the J14 cell line ideal for mutational analysis and introduction of reporter constructs.

Source:
J14 cells are a clonal derivative of Jurkat, a T cell line derived from bone marrow of a 14-year old male (4). Jurkat cells were transfected with an episomal vector and selected for hygromycin resistance. The transgene was subsequently lost via non-selective growth (2).

T-cell-receptor signaling has a critical role in adaptive immune responses and has been extensively studied as a general model for complex cellular responses (1). T-cell-receptor signaling to downstream pathways is mediated by non-receptor protein tyrosine kinases (PTKs), which interact with substrate proteins in a complex network.

References:
1. J Cell Sci. 2009; 122(Pt 9): 1269-1273.
2. J Biol Chem. 1995; 270(13): 7029-7032.
3. Science. 1998; 281(5375): 413-416.
4. Int J Cancer. 1977; 19(5): 621-626.

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Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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