5.09887
miR-372/3 Inhibitor, Neomycin-S
Synonym(s):
miR-372/3 Inhibitor, Neomycin-S, miRNA-372 Inhibitor, miRNA-373 Inhibitor
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About This Item
Empirical Formula (Hill Notation):
C39H60N12O14S
Molecular Weight:
953.03
NACRES:
NA.77
UNSPSC Code:
12352200
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
color
off-white
solubility
DMSO: 100 mg/mL
storage temp.
−70°C
Quality Level
Related Categories
Biochem/physiol Actions
Primary Target
miRNA-272/273
miRNA-272/273
Reversible: yes
Disclaimer
Toxicity: Standard Handling (A)
General description
A cell-permeable neomycin derived artificial nucleobase conjugate that inhibits the production of oncogenic miRNAs 372 (IC50 = 2.4 µM; Kd = 16 nM) and 373 (IC50 = 5.13 µM Kd = 21.4 nM) by directly binding to their precursors pre-miRNAs and interrupts their processing by Dicer. In comparison, neomycin does not affect these miRNAs (IC50 = 125 µM; Kd = 7.4 µM). Shown to depress proliferation of AGS gastric cancer cells expressing miRNA 372 and 373. However, it has no effect on MKN74 gastric epithelial cells that do not express miRNA 372 and 373. Reduced levels of miRNA 372 results in de-repression of their targets, such as the cell cycle regulator large tumor suppressor homologue 2 (LATS2).
Please note that the molecular weight for this compound is batch-specific due to variable water content.
Please note that the molecular weight for this compound is batch-specific due to variable water content.
Other Notes
Vo, D.D., et al. 2014. ACS Chem. Biol.9, 711.
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-2-°C). Stock solutions are stable for up to 3 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Duc Duy Vo et al.
ACS chemical biology, 9(3), 711-721 (2013-12-24)
MicroRNAs (miRNAs) are a recently discovered category of small RNA molecules that regulate gene expression at the post-transcriptional level. Accumulating evidence indicates that miRNAs are aberrantly expressed in a variety of human cancers and revealed to be oncogenic and to
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