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04-822

Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

culture supernatant, clone 3L74, Upstate®

Synonym(s):

ras homolog

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3L74, monoclonal
Application:
WB
Citations:
12
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biological source

rabbit

Quality Segment

100

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

3L74, monoclonal

species reactivity

rat, human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_001664

UniProt accession no.

P61586

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... RHOA(387)

General description

Mr 22kDa
Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking.

Immunogen

KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility

Application

Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
This Anti-Rho (-A Antibody, -B, -C), clone 3L74 is validated for use in WB for the detection of Rho (-A, -B, -C).
Western Blot Analysis:
A 1:1000-1:4000 dilution of this lot detected Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. This antibody also recognized recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins.

Biochem/physiol Actions

Broad species cross-reactivity expected based on sequence homology
Recognizes Rho (-A, -B, -C)

Physical form

Cultured supernantant in 0.05% sodium azide

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

routinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells

Other Notes

Replaces: 05-822

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

04-822:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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