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05-831-I

Anti-Beta (β)-Amyloid antibody

mouse monoclonal, 12F4

Synonym(s):

Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
12F4, monoclonal
Species reactivity:
human
Application:
ELISA, IHC
Citations:
8
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Product Name

Anti-Amyloid Beta (ABeta) x-42 Antibody, clone 12F4, clone 12F4, from mouse

biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

12F4, monoclonal

species reactivity

human

technique(s)

ELISA: suitable, immunohistochemistry: suitable

isotype

IgG1κ

NCBI accession no.

NP_000475

UniProt accession no.

P05067

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... APP(351)

General description

4 kDa calculated
One of the most important and initial steps which causes loss of memory and cognition in Alzheimer’s Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (Amyloid Beta) 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.

Immunogen

KLH-conjugated linear peptide corresponding to human Amyloid Beta (ABeta) x-42.

Application

Anti-Amyloid Beta (ABeta) x-42, clone 12F4 is an antibody targeting the Amyloid Beta (ABeta) x-42 protein, validated for use in IHC & ELISA.
Immunohistohemistry Analysis: A 1:500 dilution from a representative lot detected Amyloid Beta (ABeta) x-42 in human pons/midbrain tissue.

ELISA Analysis: A representative lot detected Amyloid Beta (ABeta) x-42, but demonstrates a loss of signal against Amyloid Beta (ABeta) x-42.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Amyloid Beta (ABeta) x-42, but demonstrated a loss of signal against x-40 and not x-43 in WB (Prof. J. Buxbaum, Mt. Sinai School of Medicine).

Biochem/physiol Actions

This antibody detects Amyloid Beta (ABeta) x-42, but does not cross react with Amyloid Beta (ABeta) x-40 or Amyloid Beta (Abeta) x-43.

Physical form

Format: Purified

Analysis Note

Control
Human Alzheimer′s brain tissue.
Evaluated by Immunohistochemistry in human Alzheimer′s disease brain tissue.

Immunohistochemistry Analysis: A 1:2,000 dilution of this antibody detected Amyloid Beta (Abeta) x-42 in human Alzheimer′s disease brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 05-831

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

05-831-I:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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