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1.10266

Lauryl Sulfate Broth

GranuCult® prime, according to ISO 4831, ISO 7251, FDA-BAM, SMWWPart 9221, GB 4789.38-2012, granular, for Escherichia coli, pkg of 5 kg, pkg of 500 g

Synonym(s):

Coliform selective medium, Lauryl Sulfate broth (Granulated)

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106213
Manufacturer/tradename:
GranuCult® prime
Agency:
SMWWPart 9221, FDA-BAM, GB 4789.38-2012, ISO 7251, according to ISO 4831
Ph:
6.8 (35.5 g/L in H2O), 6.8±0.2 (25 °C)
Form:
granular
Suitability:
Escherichia coli
Application(s):
food and beverages
microbiology
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Quality Level

200

agency

SMWWPart 9221, FDA-BAM, GB 4789.38-2012, ISO 7251, according to ISO 4831

sterility

non-sterile

form

granular

packaging

pkg of 5 kg, pkg of 500 g

manufacturer/tradename

GranuCult® prime

storage condition

dry media (Keep tightly closed)

technique(s)

microbiological culture: suitable

color

beige

pH

6.8 (35.5 g/L in H2O), 6.8±0.2 (25 °C)

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Escherichia coli

General description

GranuCult® prime Lauryl Sulfate broth (acc. ISO 4831, ISO 7251, and FDA-BAM) is used to selectively enrich and detect coliforms from food and animal feed, water, and other materials. The high nutrient quality and the presence of phosphate buffer ensure rapid growth and increased gas production of even the "slowly lactose-fermenting" coliform bacteria. Gas formation can be detected by using Durham tubes. The lauryl sulfate inhibits the growth of undesired bacteria.

Application

GranuCult® prime Lauryl Sulfate broth is suitable for the selective enrichment and presumptive detection of coliform bacteria from food and animal feed, water, and other materials.

Features and Benefits

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): >10⁴
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): >10⁴
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): +
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): +
Growth (Citrobacter freundii ATCC 43864 (WDCM 00006)): +
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): none
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): none
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Escherichia coli ATCC 8739 (WDCM 00012)): +
Gas (Citrobacter freundii ATCC 43864 (WDCM 00006)): +
Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)): -
Gas (Enterococcus faecalis ATCC 29212 (WDCM 00087)): -
Incubation:
Escherichia coli: 24 ± 2 hours at 30 ± 1 °C and parallel 24 ± 2 hours at 37 ± 1 °C
Citrobacter: 24 ± 2 hours at 30 ± 1 °C
Enterococcus faecalis: 48 ± 2 hours at 30 ± 1 °C and parallel 48 ± 2 hours at 37 ± 1 °C

Other Notes

  • The prepared medium is clear and yellowish-brown
  • Store the prepared medium in screw-capped containers at 2 °C to 8 °C in the dark and for up to one month
Replaces: 1.10266.5007

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

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Storage Class

11 - Combustible Solids

wgk

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

1102660214: + 1102660507: + 1.10266.0201: + 1102660000: + 1102665007: + 1102660500: + 1102665000:

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Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
1.10266.500004022536123219
1.10266.050004022536123189