Skip to Content
Merck

Key Documents

View All Documentation

324708

Asp-N Protease

Cleaves N-terminal to aspartic acid residues, suitable for Mass Spectrometry, from Pseudomonas fragi mutant strain

Synonym(s):

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

CAS Number:
9001-92-7
EC Number:
3.4.24.33 (BRENDA, IUBMB)
MDL number:
MFCD00004464
NACRES:
NA.54
UNSPSC Code:
12352202
eCl@ss:
32160410
Specific activity:
≥20,000 units/mg protein
Assay:
≥90% (SDS-PAGE)
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi,

grade

Proteomics Grade

Quality Segment

100

assay

≥90% (SDS-PAGE)

form

lyophilized solid

specific activity

≥20,000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

General description

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Physical form

Lyophilized from 10 mM Tris-HCl, pH 7.5.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Other Notes

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.
One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Still not finding the right product?

Explore all of our products under Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

324708-UG: + 324708-2UG:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library